P-112: Kinetic Evaluation of Buffalo Epididymal Spermatozoa Preserved in Different Culture Media

Background: Epididymal sperm has been recovered with the aim of conserving potentially valuable genetics Materialss from post mortem males for subsequent usage in assisted reproduction technologies. Among the quality parameters of spermatozoa, motility and velocity patterns are sensitive for chemical and physical components of medium which they were diluted. Also close correlation between sperm motion characteristics and sperm fertility has been found. So the aim of this study was to evaluate, motion characteristics of buffalo epididymal spermatozoa preserved in different culture media during 6 hour incubation. Materials and Methods: Thirty testes from 15 mature buffalo bulls (3-5 year) were collected immediately after animal’s death from Urmia slaughterhouse and transported to laboratory in cool condition. Spermatozoa were collected from caudal epididymis and pooled together. The samples were diluted in Ham’s F10, human tubal fluid (HTF), Tissue culture medium (TCM) with 7.2 PH and concentration of 20×106 sperm/ml. sperm motion characteristics were evaluated 1, 3 and 6 hours of incubation at 37 °C with computer assisted sperm analyzer(CASA HFT version 6.0). Results: Result showed that, all of the kinetics parameters reduced dramatically in time depend manner. Preservation of epididymal sperm in HTF and TCM media yielded higher motility and progressive motility as compared with Ham’s F10 at 1, 3 and 6 hours of preservation (p<0.05). Velocity patterns (straight line, curvilinear and average path velocity) were highest in HTF medium and lowest in Ham’sF10 medium in all of the incubation time (p<0.05). Conclusion: It can conclude that preservation of buffalo epididymal spermatozoa in HTF medium at 37°C seems to be suitable for sperm motility and velocity patterns.